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capn2 60942 overexpression constructs  (Addgene inc)


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    Addgene inc capn2 60942 overexpression constructs
    Fig. 5. MATR3 is a substrate for CAPN1, and the pathogenic S85C mutation renders it resistant to degradation. Transfection of HEK293T cells with calpain expressing constructs resulted in robust expression of CAPN1 (A) and <t>CAPN2</t> (B) compared to controls. (C and D) CAPN1 but not CAPN2 overexpression resulted in MATR3 degradation (n = 3; *P = 0.010; one-way ANOVA with Tukey’s post hoc test). (E and F) While exogenous FLAG-MATR3(WT) is susceptible to cleavage by CAPN1 (n = 3; *P = 0.036; ns, not significant; one-way ANOVA with Tukey’s post hoc test), the pathogenic S85C mutation is resistant (n = 3; one- way ANOVA with Tukey’s post hoc test).
    Capn2 60942 Overexpression Constructs, supplied by Addgene inc, used in various techniques. Bioz Stars score: 90/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/capn2+60942+overexpression+constructs/p3XFlag-CAPN2+(Plasmid+%2360942)/pm37011198-253-3-10
    Average 90 stars, based on 6 article reviews
    capn2 60942 overexpression constructs - by Bioz Stars, 2026-09
    90/100 stars

    Images

    1) Product Images from "Neuronal activity regulates Matrin 3 abundance and function in a calcium-dependent manner through calpain-mediated cleavage and calmodulin binding."

    Article Title: Neuronal activity regulates Matrin 3 abundance and function in a calcium-dependent manner through calpain-mediated cleavage and calmodulin binding.

    Journal: Proceedings of the National Academy of Sciences of the United States of America

    doi: 10.1073/pnas.2206217120

    Fig. 5. MATR3 is a substrate for CAPN1, and the pathogenic S85C mutation renders it resistant to degradation. Transfection of HEK293T cells with calpain expressing constructs resulted in robust expression of CAPN1 (A) and CAPN2 (B) compared to controls. (C and D) CAPN1 but not CAPN2 overexpression resulted in MATR3 degradation (n = 3; *P = 0.010; one-way ANOVA with Tukey’s post hoc test). (E and F) While exogenous FLAG-MATR3(WT) is susceptible to cleavage by CAPN1 (n = 3; *P = 0.036; ns, not significant; one-way ANOVA with Tukey’s post hoc test), the pathogenic S85C mutation is resistant (n = 3; one- way ANOVA with Tukey’s post hoc test).
    Figure Legend Snippet: Fig. 5. MATR3 is a substrate for CAPN1, and the pathogenic S85C mutation renders it resistant to degradation. Transfection of HEK293T cells with calpain expressing constructs resulted in robust expression of CAPN1 (A) and CAPN2 (B) compared to controls. (C and D) CAPN1 but not CAPN2 overexpression resulted in MATR3 degradation (n = 3; *P = 0.010; one-way ANOVA with Tukey’s post hoc test). (E and F) While exogenous FLAG-MATR3(WT) is susceptible to cleavage by CAPN1 (n = 3; *P = 0.036; ns, not significant; one-way ANOVA with Tukey’s post hoc test), the pathogenic S85C mutation is resistant (n = 3; one- way ANOVA with Tukey’s post hoc test).

    Techniques Used: Mutagenesis, Transfection, Expressing, Construct, Over Expression

    Related Articles

    Over Expression:

    Article Title: Neuronal activity regulates Matrin 3 abundance and function in a calcium-dependent manner through calpain-mediated cleavage and calmodulin binding.
    Article Snippet: .. CAPN1 (#60941) and CAPN2 (#60942) overexpression constructs were purchased from Addgene. .. Expression plasmids for pGW1 FLAG-MATR3(WT) and FLAGMATR3(S85C) were generated by amplifying MATR3(WT) and MATR3(S85C) from pGW1 FLAG-MATR3-EGFP constructs (51) using Q5 Hot Start High-Fidelity DNA Polymerase (New England Biolabs) and flanking primers that eliminated linker Na+ Ca2+ Ca2+ entry CaM CAPN1CaM activation CAPN1 activation MATR3 degradation by CAPN1 RNA-bound MATR3 RNA release NMDAR ZF1 ZF2RRM1 RRM2MATR3 Ca2+/CaM binding to RRM2 pathogenic S85C mutation Fig. 8.

    Article Title: Neuronal activity regulates Matrin 3 levels and function in a calcium-dependent manner through calpain cleavage and calmodulin binding
    Article Snippet: .. CAPN1 (#60941) and CAPN2 (#60942) overexpression constructs were purchased from Addgene. .. Expression plasmids for pGW1 FLAG-MATR3(WT) and FLAG-MATR3(S85C) were generated by amplifying MATR3(WT) and MATR3(S85C) from pGW1 FLAG-MATR3-EGFP constructs using Q5 Hot Start High-Fidelity DNA Polymerase (New England Biolabs) and flanking primers that eliminated linker sequences and EGFP.

    Construct:

    Article Title: Neuronal activity regulates Matrin 3 abundance and function in a calcium-dependent manner through calpain-mediated cleavage and calmodulin binding.
    Article Snippet: .. CAPN1 (#60941) and CAPN2 (#60942) overexpression constructs were purchased from Addgene. .. Expression plasmids for pGW1 FLAG-MATR3(WT) and FLAGMATR3(S85C) were generated by amplifying MATR3(WT) and MATR3(S85C) from pGW1 FLAG-MATR3-EGFP constructs (51) using Q5 Hot Start High-Fidelity DNA Polymerase (New England Biolabs) and flanking primers that eliminated linker Na+ Ca2+ Ca2+ entry CaM CAPN1CaM activation CAPN1 activation MATR3 degradation by CAPN1 RNA-bound MATR3 RNA release NMDAR ZF1 ZF2RRM1 RRM2MATR3 Ca2+/CaM binding to RRM2 pathogenic S85C mutation Fig. 8.

    Article Title: Neuronal activity regulates Matrin 3 levels and function in a calcium-dependent manner through calpain cleavage and calmodulin binding
    Article Snippet: .. CAPN1 (#60941) and CAPN2 (#60942) overexpression constructs were purchased from Addgene. .. Expression plasmids for pGW1 FLAG-MATR3(WT) and FLAG-MATR3(S85C) were generated by amplifying MATR3(WT) and MATR3(S85C) from pGW1 FLAG-MATR3-EGFP constructs using Q5 Hot Start High-Fidelity DNA Polymerase (New England Biolabs) and flanking primers that eliminated linker sequences and EGFP.



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    capn2 60942 overexpression constructs  (Addgene inc)
    90
    Addgene inc capn2 60942 overexpression constructs
    Fig. 5. MATR3 is a substrate for CAPN1, and the pathogenic S85C mutation renders it resistant to degradation. Transfection of HEK293T cells with calpain expressing constructs resulted in robust expression of CAPN1 (A) and <t>CAPN2</t> (B) compared to controls. (C and D) CAPN1 but not CAPN2 overexpression resulted in MATR3 degradation (n = 3; *P = 0.010; one-way ANOVA with Tukey’s post hoc test). (E and F) While exogenous FLAG-MATR3(WT) is susceptible to cleavage by CAPN1 (n = 3; *P = 0.036; ns, not significant; one-way ANOVA with Tukey’s post hoc test), the pathogenic S85C mutation is resistant (n = 3; one- way ANOVA with Tukey’s post hoc test).
    Capn2 60942 Overexpression Constructs, supplied by Addgene inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/capn2+60942+overexpression+constructs/p3XFlag-CAPN2+(Plasmid+%2360942)/pm37011198-253-3-10
    Average 90 stars, based on 1 article reviews
    capn2 60942 overexpression constructs - by Bioz Stars, 2026-09
    90/100 stars
    		  Buy from Supplier

    Image Search Results


    Fig. 5. MATR3 is a substrate for CAPN1, and the pathogenic S85C mutation renders it resistant to degradation. Transfection of HEK293T cells with calpain expressing constructs resulted in robust expression of CAPN1 (A) and CAPN2 (B) compared to controls. (C and D) CAPN1 but not CAPN2 overexpression resulted in MATR3 degradation (n = 3; *P = 0.010; one-way ANOVA with Tukey’s post hoc test). (E and F) While exogenous FLAG-MATR3(WT) is susceptible to cleavage by CAPN1 (n = 3; *P = 0.036; ns, not significant; one-way ANOVA with Tukey’s post hoc test), the pathogenic S85C mutation is resistant (n = 3; one- way ANOVA with Tukey’s post hoc test).

    Journal: Proceedings of the National Academy of Sciences of the United States of America

    Article Title: Neuronal activity regulates Matrin 3 abundance and function in a calcium-dependent manner through calpain-mediated cleavage and calmodulin binding.

    doi: 10.1073/pnas.2206217120

    Figure Lengend Snippet: Fig. 5. MATR3 is a substrate for CAPN1, and the pathogenic S85C mutation renders it resistant to degradation. Transfection of HEK293T cells with calpain expressing constructs resulted in robust expression of CAPN1 (A) and CAPN2 (B) compared to controls. (C and D) CAPN1 but not CAPN2 overexpression resulted in MATR3 degradation (n = 3; *P = 0.010; one-way ANOVA with Tukey’s post hoc test). (E and F) While exogenous FLAG-MATR3(WT) is susceptible to cleavage by CAPN1 (n = 3; *P = 0.036; ns, not significant; one-way ANOVA with Tukey’s post hoc test), the pathogenic S85C mutation is resistant (n = 3; one- way ANOVA with Tukey’s post hoc test).

    Article Snippet: CAPN1 (#60941) and CAPN2 (#60942) overexpression constructs were purchased from Addgene.

    Techniques: Mutagenesis, Transfection, Expressing, Construct, Over Expression